Maturation by LctT is required for biosynthesis of full-length lantibiotic lacticin 481.
نویسندگان
چکیده
In lantibiotic lacticin 481 biosynthesis, LctT cleaves the precursor peptide and exports mature lantibiotic. Matrix-assisted laser desorption ionization-time of flight mass spectrometry revealed that a truncated form of lacticin 481 is produced in the absence of LctT or after cleavage site inactivation. Production of truncated lacticin 481 is 4-fold less efficient, and its specific activity is about 10-fold lower.
منابع مشابه
In vitro reconstitution and substrate specificity of a lantibiotic protease.
Lacticin 481 is a lanthionine-containing bacteriocin (lantibiotic) produced by Lactococcus lactis subsp. lactis. The final steps of lacticin 481 biosynthesis are proteolytic removal of an N-terminal leader sequence from the prepeptide LctA and export of the mature lantibiotic. Both proteolysis and secretion are performed by the dedicated ATP-binding cassette (ABC) transporter LctT. LctT belongs...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 71 1 شماره
صفحات -
تاریخ انتشار 2005